Flow Cytometry Core Facility (FCCF) / (SCCC)

Overview of the Core.

 

The Flow Cytometry Core Facility (FCCF) at the Sylvester Comprehensive Cancer Center (SCCC) provides researchers with sophisticated methods for the analysis and preparative sorting of both normal and tumor cells. The facility helps researchers measure apoptosis, gene expression, drug metabolism, immune responses, and pathways of cellular activation, both normal and tumor-related.

The core maintains instruments, computer work-stations for data analysis, and related equipment to support scientists affiliated with Sylvester’s multidisciplinary research programs, as well as researchers in a wide variety of cancer-related research projects.

The facility also provides training to investigators in flow cytometric applications and cell sorting technologies.

 

Applications.

 

Potential applications of this technology include:-

  • Complex 19 fluorescence parameter immuno-phenotyping
  • DNA content, ploidy and cell cycle analysis
  • Up to 17 fluorescence parameter cell sorting and single-cell flow cloning
  • High speed (10,000 – 50,000+ cells per second) and efficiency (95%+ purity) cell sorting
  • Assessment of apoptosis
  • Molecular cytogenetics
  • Cell function studies
  • Micro-biological applications
  • Multiplexed and micro-particle-based analysis

 

Technologies.

 

  • BD FACS. Canto-II, 8-color, flow cytometric analyzer
  • BD LSR-II, 10-color, flow cytometric analyzer
  • BD LSR-Fortessa-HTS, 19-color, high-throughput, flow cytometric analyzer
  • BD FACS Aria-I, 8-color, flow cytometric cell sorter
  • BD FACS Aria-IIu, 17-color, flow cytometric cell sorter, housed in a Baker BioProtect-II, BSL-2 bio-safety cabinet
  • BD FACS Aria-II, 10 color, flow cytometric cell sorter
  • Zeiss fluorescence microscope
  • Invitrogen Countess cell counter
  • Partec CyFlow Space, 6-color flow cytometric analyser and absolute cell counter

 

 Meet the Team.

 

Huw S. (“Zip”) Kruger Gray, Ph.D., Core Facility Director

Shannon Saigh, Ph.D., Research Associate

Jay Enten, B.S., Research Associate

Patricia Guevara, B.S., M.A., Research Associate

 

 

Location and Hours of Operation.

Hours Location

Cell Sorting

11:00am - 5:00pm M-F   

 

Cell Analysis

24/7 for trained users               
9:00am - 5:00pm M-F for untrained users

Rosenstiel Medical Sciences Building 
1600 N.W. 10 Avenue
Room 7147
Miami, FL 33136

Phone: (305) 243-5571

 Links and Resources.

  1. Core Website
  2. Policies and Procedures
  3. Free Cytospec data analysis software
  4. Flow Cytometry ListServ (for exchange of information between facility users and staff)
  5. Billing Authorization Form

 

Contacts

Name Role Phone Email Location
Huw S. (“Zip”) Kruger Gray, Ph.D.
Core Director
 
(305) 243-5019
 
hkgray@med.miami.edu
 
Rosenstiel Medical Sciences Building, Room 7147, Locator R-127
 
Shannon Saigh, Ph.D.
Research Associate
 
(305) 243-5571
 
sopiela@med.miami.edu
 
Rosenstiel Medical Sciences Building, Room 7147, Locator R-127
 
Jay Enten, B.S.
Research Associate
 
(305) 243-5571
 
jenten@med.miami.edu
 
Rosenstiel Medical Sciences Building, Room 7147, Locator R-127
 
Patricia Guevara, B.S., M.A.
Research Associate
 
(305) 243-5571
 
pguevara@med.miami.edu
 
Rosenstiel Medical Sciences Building, Room 7147, Locator R-127
 

Map