Flow Cytometry Shared Resource (FCSR) / (SCCC)

Overview of the Core.


The Flow Cytometry Shared Resource (FCSR) at the Sylvester Comprehensive Cancer Center (SCCC) provides researchers with sophisticated methods for the analysis and preparative sorting of both normal and tumor cells. The facility helps researchers measure apoptosis, gene expression, drug metabolism, immune responses and pathways of cellular activation, both normal and tumor-related.

The shared resource maintains instruments, computer work-stations for data analysis and related equipment to support scientists affiliated with Sylvester’s multidisciplinary research programs, as well as researchers in a wide variety of cancer-related research projects.

The facility also provides training to investigators in flow cytometric applications, mass cytometry and cell sorting technologies.




Potential applications of this technology include:-

  • Complex 19 fluorescence parameter immuno-phenotyping.
  • DNA content, ploidy and cell cycle analysis.
  • Up to 17 fluorescence parameter cell sorting and single-cell flow cloning.
  • High speed (10,000 – 50,000+ cells per second) and efficiency (95%+ purity) cell sorting.
  • Assessment of apoptosis.
  • Molecular cytogenetics.
  • Cell function studies.
  • Micro-biological applications.
  • Multiplexed and micro-particle-based analysis.




  • BD FACS Canto-II, 8-color, flow cytometric analyzer  (instrument configuration).
  • BD LSR-II, 15-color, flow cytometric analyzer  (instrument configuration).
  • BD LSR-Fortessa-HTS, 18-color, high-throughput, flow cytometric analyzer (instrument configuration).
  • BD FACS Aria Fusion, 15-color, flow cytometric cell sorter, BSL-2 bio-safety cabinet (instrument configuration).
  • BD FACS Aria-IIu, 17-color, flow cytometric cell sorter, housed in a Baker BioProtect-II, BSL-2 bio-safety cabinet (instrument configuration).
  • BD FACS Aria-II, 13 color, flow cytometric cell sorter  (instrument configuration).
  • Cytek Aurora, 5 lasers, 3 scatter channels and 64 fluorescence channels, spectral analyzer. 
  • CyTOF - Helios for high dimensional data acquisition of cells in suspension.
  • CyTOF - Hyperion for high dimensional imaging of fresh frozen or formalin fixed parafin embedded tissues on microscope slides.
  • Zeiss fluorescence microscope.
  • Invitrogen Countess cell counter.


       CYTOF Helios Staining Protocols:


 Meet the Team.


Huw S. (“Zip”) Kruger Gray, Ph.D., Core Facility Director

Shannon Saigh, Ph.D., Research Associate

Natasha Ward, Ph.D., Research Associate

Patricia Guevara, M.A., Sr. Research Associate

Jay Enten, B.S., Research Associate



Location and Hours of Operation.

Hours Location

Cell Sorting

11:00am - 5:00pm M-F   


Cell Analysis

24/7 for trained users               
9:00am - 5:00pm M-F for untrained users

Rosenstiel Medical Sciences Building 
1600 N.W. 10 Avenue
Room 7147
Miami, FL 33136

Phone: (305) 243-5571

 Links and Resources.

  1. Core Website
  2. Policies and Procedures
  3. Free Cytospec data analysis software
  4. Flow Cytometry ListServ (for exchange of information between facility users and staff)
  5. Billing Authorization Form 
  6. New Standard Operating Procedures


Name Role Phone Email Location
Huw S. (“Zip”) Kruger Gray, Ph.D.
Core Director
(305) 243-5019
Rosenstiel Medical Sciences Building, Room 7147, Locator R-127
Shannon Saigh, Ph.D.
Research Associate
(305) 243-5571
Rosenstiel Medical Sciences Building, Room 7147, Locator R-127
Jay Enten, B.S.
Research Associate
(305) 243-5571
Rosenstiel Medical Sciences Building, Room 7147, Locator R-127
Patricia Guevara, M.A.
Sr. Research Associate
(305) 243-5571
Rosenstiel Medical Sciences Building, Room 7147, Locator R-127
Natasha Ward, Ph.D.
Research Associate
(305) 243-5571
Rosenstiel Medical Sciences Building, Room 7147, Locator R-127