Onco-Genomics Shared Resource - OGSR

Overview

 

The Onco-Genomics Shared Resource (OGSR) is a state-of-the-art open-core facility located in the Papanicolaou Cancer Research Building (PAP) on the University of Miami, Miller School of Medicine campus. The OGSR provides Sylvester investigators access to a wide range of technologies and services for the study of the genomics and epigenetics of cancer. Principle service areas encompass: 1) extensive services for next generation sequencing (NGS), 2) single cell genomics 3) gene expression assays and molecular quantitation, 4) services for sample extraction and QC.

The OGSR is a component of the NCI Sylvester Cancer Center Support Grant (P30 CA240139).

 

Instrumentation includes:

 

Instrument Description
Illumina NovaSeq 6000 Production scale, short read NGS sequencer
Illumina NextSeq 500 Rapid run, benchtop, short read NGS sequencer
Illumina iSeq 100 Low output benchtop, short read NGS sequencer
PE Zephyr G3 NGS Automated liquid handler for NGS library prep
UVP UV3 HEPA cabs (two) HEPA filtered UV cabinets for critical PCR/amplification
10x Genomics Chromium Single cell controller for genomics assays
Mission Bio Tapestri Single cell controller for genomics assays
Nexcelom K2 Imaging cell counter with 2 channel fluorescence
Agilent FA 5200 12 capillary high-throughput DNA/RNA fragment analyzer
Agilent BioAnalyzer 2100 Lab-on-chip DNA/RNA fragment analyzer
Thermo Qubit 3.0 Single tube fluorimeter for dye-based DNA/RNA quantification
Tecan Infinite F200 Microplate reader, single channel fluorescence
Bio-Rad C1000 (two) Thermal cyclers with deep-well and standard 96-well blocks
Eppendorf MasterCycler Thermal cycler, 96-well with vapor-protect lid (Pro S model)
nanoString MAX nanoString PrepStation and nCounter scanner (2nd generation)
RainDance RainDrop Source and Sense instruments for droplet digital PCR (ddPCR)
Covaris CryoPrep CP02 Cryo-fractionation impactor for 0.05-10 g samples
Thermo NanoDrop 8000 Micro-volume droplet spectrophotometer

 

Location and hours of operation

Hours Location 

Mon-Fri 9:00AM - 5:00PM 

Onco-Genomics Shared Resource (OGSR)

PAP 414
1550 N.W. 10th Avenue
Miami, FL 33136

Links and Resources

Telephone: 305 243 7927 

Email: OGSR@miami.edu

 

Map