Oncogenomics Core Facility (OCF)

Overview

 

The Oncogenomics Core Facility (OCF) is a state-of-the-art open-core facility located in the in the Biomedical Research Building on the University of Miami, Miller School of Medicine campus. The OCF provides investigators access to a wide range of technologies and services for the molecular analysis of DNA and RNA in addition to providing technical expertise for project design and trouble-shooting. The OCF employs four full-time technicians overseen by Faculty-level Directors. Services span RNA/DNA extraction and QC, droplet digital-PCR, PCR-free gene expression assays, single-cell sample prep and gene expression analysis, miRNA and CNV assays, microarrays and rapid run next generation sequencing for RNA-Seq, ChIP-Seq and other NGS workflows. OCF facilities, instrumentation and bench space, are divided into sample processing (pre-PCR) and analytical laboratories (post-PCR) in two separate open-plan laboratories. All equipment is maintained under rolling service contracts with regular preventative maintenance.

 

OCF instrumentation includes:

  • NanoString PrepStation and nCounter analysis systems
  • Illumina NextSeq 500 sequencer
  • Raindance Technologies Raindrop droplet digital ddPCR system
  • BioRad QX200 droplet digital ddPCR system
  • Fluidigm C1 single-cell auto-prep station and Biomark HD nanofluidic qPCR system
  • Two Roche Lightcycler LC480 real-time PCR machines fed by a PE/Caliper Twister II autoloader and Mécour refrigerated sample storage system
  • Bio-Rad C1000 deep-well and Eppendorf Mastercycler Pro-S PCR systems
  • Thermo Scientific Nanodrop, LifeTechnologies Qubit and Agilent Bioanalyzer 2100 systems
  • Eppendorf epMotion 5075 and Beckman Coulter Biomek NXP automation systems

 

Leadership

 

Ramin Shiekhattar, Ph.D., Academic Director

 

Ramin Shiekhattar, Ph.D., joined the Sylvester Comprehensive Cancer Center in 2014 as Chief of the Division of Cancer Genomics and Epigenetics and Director of the Cancer Epigenetics Research Program. Dr. Shiekhattar is the Academic Director of the OCF and has led the expansion of OCF services to meet the changing needs of investigators at the Sylvester Comprehensive Cancer Center and in particular the needs of the Cancer Epigenetics Research Program. 

 

Siôn Williams, Ph.D., Core Director

 

Siôn Williams, Ph.D., is the Director of the Oncogenomics Core Facility (OCF) and has led the facility since 2013. Dr. Williams is a Co-Director of the CFAR Laboratory Core and a Research Assistant Professor in the Department of Neurology. 

 

 

Location and hours of operation

Hours Location & Mailing Address

Mon-Fri 8:00AM - 5:00PM 

Oncogenomics Core Facility

Biomedical Research Building (BRB) 542C
1501 N.W. 10th Avenue
Miami, FL 33136

Links and Resources

 

Telephone: 305 243 7927 

 

OCF staff member Contact: Email:
Siôn Williams - Director slwilliams@miami.edu
Corneliu Sologon - NGS library prep and sequencing  csologon@miami.edu
Yoslayma Cardentey - NanoString, Exiqon qPCR and sample prep ycardentey@miami.edu
Robert Suter ddPCR and  rks77@miami.edu
     

  

  

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